Identification of locally isolated entomopathogenic Fusarium species from the soil of Changa Manga Forest, Pakistan and evaluation of their larvicidal efficacy against Aedes aegypti

Abstract Dengue fever vectored by the mosquito Aedes aegypti is one of most rapidly spreading insect-borne diseases. Current reliance dengue vector control mostly on chemical insecticides. Growing insecticide resistance in primary vector, aegypti, limits effectiveness through These insecticides also have negative environmental impacts animals, plants and human health. Myco-biocontrol agents are naturally occurring organisms found to be less damaging environment as compared In present study, entomopathogenic potential local strains fungi isolated from soil was assessed for vector. Local fungal isolates presents better alternative introducing a foreign biocontrol strain, they may adapted conditions area survive more efficacy against target organism. Larvicidal Fusarium equiseti proliferatum evaluated aegypti. F. (MK371718) (MK371715) were Changa Manga Forest, Pakistan using insect bait method. activity two spp. tested forth instar larvae A. laboratory, concentrations 105, 106, 107 108 conidia /ml. LC50 values after 24h, 48h, 72h 96h exposure recorded 3.8x 108, 2.9x107, 2.0x107, 7.1x106 /ml respectively while 1.21x108, 9.6x107, 4.2x107, 2.6x107 exposure. The results indicate that among effective terms its larvicidal than